Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures Free Download: Radioimmunoassay (RIA) is a very sensitive in vitro assay for detecting the presence of an antigen. Basically, any biological molecule for which an antibody exists may be tested, even at extremely low quantities. The target antigen is radioactively tagged and coupled to its particular antibodies.

Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures

Then a sample, such as blood serum, is introduced to start a competitive response between the labeled antigens from the preparation and the unlabeled antigens from the serum-sample with the particular antibodies. 125-I labels are most commonly employed, however other isotopes such as C14 and H3 have also been utilized. To separate bind and free radio-labeled antigen, double antibody, charcoal, cellulose, chromatography, or solid phase methods are used.

Table of Content

  • Definition
  • Introduction
  • RIA Requirements
  • RIA Principles
  • RIA Procedures
  • RIA Applications
  • RIA Advantages
  • RIA Limitations
  • Conclusion

Radioimmunoassay PPT: Definition, Requirements, Principles and Procedures

Sumit ThakurGeneral Seminar TopicsRadioimmunoassay PPT: Definition, Requirements, Principles and Procedures Free Download: Radioimmunoassay (RIA) is a very sensitive in vitro assay for detecting the presence of an antigen. Basically, any biological molecule for which an antibody exists may be tested, even at extremely low quantities. The target antigen is radioactively tagged and...